ALK5

• Description :

  Most cell types express three sizes of receptors for TGFβ. These are designated Type I (53 kDa), Type II (7085 kDa), and Type III (250-350 kDa) . The Type III receptor, a proteoglycan that exists in membranebound and soluble forms, binds TGFβ1, TGFβ2, and TGFβ3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane bound serine/threonine kinase that binds TGFβ1 and TGFβ3 with high affinity and TGFβ2 with a much lower affinity. The Type I receptor, originally known as ALK5 (Activin receptor-like kinase) is also a membranebound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGFβ. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

• Synonyms :

  Tgfbr1; ALK5; Alk-5; TbetaRI; AU017191; TbetaR-I

• NCBI Gene ID :

  21812

• UniProt :

  Q64729

• Accession Number :

  NP_033396.1

• Accession Number mRNA :

  NM_009370.2

• Chromosomal Location :

  4 B1; 4 19.3 cM

• Reactivity :

  Anti-Mouse

• Cross Reactivity :

  Mouse

• Target Antigen :

  Recmobinant mouse ALK5 extracellular domain

• Clone :

  (#3A11)

• Applications :

  WB, IHC

• Purification Method :

  Protein G chromatography

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  PBS

• Storage Conditions :

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source :

  Rat

• Isotype :

  IgG2