ALK5

• Description:

  Most cell types express three sizes of receptors for TGFβ. These are designated Type I (53 kDa), Type II (7085 kDa), and Type III (250-350 kDa) . The Type III receptor, a proteoglycan that exists in membranebound and soluble forms, binds TGFβ1, TGFβ2, and TGFβ3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane bound serine/threonine kinase that binds TGFβ1 and TGFβ3 with high affinity and TGFβ2 with a much lower affinity. The Type I receptor, originally known as ALK5 (Activin receptor-like kinase) is also a membranebound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGFβ. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

• Synonyms:

  Tgfbr1; ALK5; Alk-5; TbetaRI; AU017191; TbetaR-I

• NCBI Gene ID:

  21812

• UniProt:

  Q64729

• Accession Number:

  NP_033396.1

• Accession Number mRNA:

  NM_009370.2

• Chromosomal Location:

  4 B1; 4 19.3 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recmobinant mouse ALK5 extracellular domain

• Clone:

  (#3A11)

• Applications:

  WB, IHC

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2