Our Custom Services

Gentaur provides NGS Bioinformatics Analysis Services to support research laboratories, sequencing facilities, and biotechnology companies in converting next-generation sequencing (NGS) data into reliable, interpretable results. This service eliminates the need for in-house bioinformatics infrastructure while ensuring secure, reproducible, and scalable analysis workflows.

Supported NGS Applications

The service supports a wide range of sequencing applications, including:

1. RNA sequencing (RNA-seq).
2. Whole-genome sequencing (WGS).
3. Whole-exome sequencing (WES).
4. Targeted DNA sequencing.
5. Variant detection and annotation.

All workflows are built using validated and standardised pipelines, ensuring consistent results suitable for research reporting and downstream analysis.

Secure and Flexible Data Handling

Gentaur offers flexible data handling options adapted to institutional requirements. Analyses can be performed using secure cloud environments, including Bring Your Own Cloud (BYOC) configurations, allowing customers to maintain full control over data ownership, governance, and compliance.

This approach minimises data transfer risks while enabling efficient use of scalable computing resources.

End-to-End Scientific Support

Each project is accompanied by dedicated customer and scientific support, including:

1. Pre-analysis consultation and workflow selection
2. Assistance with data preparation and upload
3. Technical support during analysis execution
4. Guidance on result interpretation and reporting

This ensures that both bench scientists and bioinformatics teams receive practical, human support throughout the project lifecycle.

Reproducibility and Reliability

All analyses are performed using reproducible, version-controlled workflows, supporting consistent results across projects and long-term studies. This is particularly valuable for collaborative research, sequencing cores, and multi-year projects.

Who This Service Is For ?

This service is suitable for :

1. Academic and clinical research laboratories
2. Sequencing core facilities
3. Biotech and pharmaceutical R&D teams
4. CROs and assay developers
5. Research groups working with large or complex NGS datasets

We provide high-precision HPLC-MS/MS (High-Performance Liquid Chromatography-Tandem Mass Spectrometry) testing solutions to support clinical diagnostics and research. Our state-of-the-art technology ensures accurate, reliable, and fast results for a range of critical biomarkers.

HPLC-MS/MS Specialized Testing Solutions:

Vitamin & Nutritional Testing

- 25-Hydroxyvitamin D2-D3 in Serum – Essential for assessing Vitamin D levels, crucial for bone health and immune function.

- Vitamin ADE in Serum – Measures vitamins A, D, and E to evaluate nutritional status and potential deficiencies.

Amino Acids & Metabolic Testing

- Amino Acids in Biological Fluids – Used for detecting metabolic disorders and nutritional imbalances.

- Organic Acids in Urine – Helps diagnose metabolic conditions, including inherited metabolic disorders.

Neonatal & Genetic Screening

- Neonatal Screening in DBS (Dried Blood Spot) – A crucial early screening for metabolic and genetic conditions in newborns.

- Phenylketonuria (PKU) Testing (DBS & Serum) – Detects PKU, a rare genetic disorder affecting metabolism.

Neurological & Neurotransmitter Testing

- Biogenic Amines in Urine – Evaluates neurotransmitter levels for conditions related to mood, stress, and nervous system health.

- Neurotransmitters in Urine – Helps assess neurological disorders, mood imbalances, and stress responses.

- VMA / HVA / 5-HIAA in Urine – Used in the diagnosis of neuroendocrine tumors, including pheochromocytoma and neuroblastoma.

Hormonal & Stress Response Markers

- Catecholamines in Urine – Measures stress-related hormones like dopamine, epinephrine, and norepinephrine.

- Metanephrine in Urine – Helps diagnose adrenal gland disorders and monitor stress-related conditions.

Immunosuppressant & Transplant Monitoring

- Immunosuppressant in Whole Blood – Critical for transplant patients to monitor medication levels and avoid rejection risks.

Gut & Metabolic Health

- Gut Dysbiosis Metabolite Markers in Urine – Evaluates microbiome imbalances, aiding in gut health and disease research.

Fatty Acid & Energy Metabolism

- Carnitine / Acylcarnitine in DBS – Assesses energy metabolism and identifies disorders affecting fatty acid oxidation.

At Gentaur, we understand that precision is everything in molecular biology. That’s why we’ve developed a cutting-edge approach to producing custom DNA ladders tailored to your exact specifications. Using advanced techniques, we create size standards that are not only highly accurate and reproducible but also optimized for performance across a wide range of applications.

Rather than relying on conventional fragment assembly, our method ensures consistency and clarity in every band — delivering the confidence you need in your gel electrophoresis results.

How We Make Your Custom DNA Ladder

At Gentaur, we don’t just mix DNA fragments — we use proven, state-of-the-art molecular biology methods to create high-quality, reliable DNA ladders designed just for your needs.

High-Fidelity PCR Amplification

We start by amplifying your specified DNA fragments using high-fidelity DNA polymerases. This ensures the fragments have exact base pair lengths with minimal errors — critical for accurate sizing in your gels.

Enzymatic Digestion & Fragment Assembly

For complex or unusual fragment patterns, we use restriction enzymes or site-specific nucleases to cut plasmid DNA at precise locations. This lets us generate DNA fragments of non-standard sizes or unique patterns tailored to your requirements.

Purification by Size-Exclusion Chromatography or Gel Extraction

Each fragment is purified to remove primer dimers, nonspecific products, and residual enzymes. We use size-exclusion chromatography or gel extraction techniques to isolate clean, size-specific DNA bands, ensuring your ladder has sharp, distinct bands on gels.

Labeling and Modification (Optional)

Need fluorescent tags, biotin, or other chemical labels on your ladder fragments? We perform post-PCR labeling or incorporate modified nucleotides during amplification to give you ladders compatible with fluorescence imaging, affinity capture, or other detection methods.

Quantification and Stoichiometric Balancing

Each fragment’s concentration is carefully measured using UV spectrophotometry and fluorometric assays. We then mix fragments at controlled ratios to produce ladders with optimized band intensities — so you get clear, easily interpretable patterns on your gels.

Quality Control & Validation

Before shipping, each batch undergoes:

1. Capillary electrophoresis for precise size confirmation
2. Spectrophotometric purity assessment to check DNA integrity
3. Gel electrophoresis testing under your requested conditions to verify ladder performance

Why This Matters for Your Research

1. Accurate fragment sizing: Your ladder matches the exact fragment sizes needed — no guesswork, no missing bands.
2. Consistent band intensity: Balanced concentrations give you clear visualization and reliable quantitation.
3. Compatibility with your detection method: Fluorescent or biotin-labeled ladders improve signal-to-noise ratio on specialized imaging platforms.
4. Reproducibility: Strict QC means every batch performs the same — so your data stays consistent over time.

What You Get

1. Custom DNA ladder with your chosen fragment sizes and band intensities
2. Optional chemical labels integrated for advanced detection
3. Ready-to-use or freeze-dried formats based on your preference
4. Full documentation including fragment map, concentration data, and QC reports
5. Expert support for integration into your protocols