Our Custom Services

At Gentaur, we understand that precision is everything in molecular biology. That’s why we’ve developed a cutting-edge approach to producing custom DNA ladders tailored to your exact specifications. Using advanced techniques, we create size standards that are not only highly accurate and reproducible but also optimized for performance across a wide range of applications.

Rather than relying on conventional fragment assembly, our method ensures consistency and clarity in every band — delivering the confidence you need in your gel electrophoresis results.

How We Make Your Custom DNA Ladder

At Gentaur, we don’t just mix DNA fragments — we use proven, state-of-the-art molecular biology methods to create high-quality, reliable DNA ladders designed just for your needs.

High-Fidelity PCR Amplification

We start by amplifying your specified DNA fragments using high-fidelity DNA polymerases. This ensures the fragments have exact base pair lengths with minimal errors — critical for accurate sizing in your gels.

Enzymatic Digestion & Fragment Assembly

For complex or unusual fragment patterns, we use restriction enzymes or site-specific nucleases to cut plasmid DNA at precise locations. This lets us generate DNA fragments of non-standard sizes or unique patterns tailored to your requirements.

Purification by Size-Exclusion Chromatography or Gel Extraction

Each fragment is purified to remove primer dimers, nonspecific products, and residual enzymes. We use size-exclusion chromatography or gel extraction techniques to isolate clean, size-specific DNA bands, ensuring your ladder has sharp, distinct bands on gels.

Labeling and Modification (Optional)

Need fluorescent tags, biotin, or other chemical labels on your ladder fragments? We perform post-PCR labeling or incorporate modified nucleotides during amplification to give you ladders compatible with fluorescence imaging, affinity capture, or other detection methods.

Quantification and Stoichiometric Balancing

Each fragment’s concentration is carefully measured using UV spectrophotometry and fluorometric assays. We then mix fragments at controlled ratios to produce ladders with optimized band intensities — so you get clear, easily interpretable patterns on your gels.

Quality Control & Validation

Before shipping, each batch undergoes:

• Capillary electrophoresis for precise size confirmation
• Spectrophotometric purity assessment to check DNA integrity
• Gel electrophoresis testing under your requested conditions to verify ladder performance

Why This Matters for Your Research

• Accurate fragment sizing: Your ladder matches the exact fragment sizes needed — no guesswork, no missing bands.
• Consistent band intensity: Balanced concentrations give you clear visualization and reliable quantitation.
• Compatibility with your detection method: Fluorescent or biotin-labeled ladders improve signal-to-noise ratio on specialized imaging platforms.
• Reproducibility: Strict QC means every batch performs the same — so your data stays consistent over time.

What You Get

• Custom DNA ladder with your chosen fragment sizes and band intensities
• Optional chemical labels integrated for advanced detection
• Ready-to-use or freeze-dried formats based on your preference
• Full documentation including fragment map, concentration data, and QC reports
• Expert support for integration into your protocols

We provide high-precision HPLC-MS/MS (High-Performance Liquid Chromatography-Tandem Mass Spectrometry) testing solutions to support clinical diagnostics and research. Our state-of-the-art technology ensures accurate, reliable, and fast results for a range of critical biomarkers.

Specialized Testing Solutions:

Vitamin & Nutritional Testing

• 25-Hydroxyvitamin D2-D3 in Serum – Essential for assessing Vitamin D levels, crucial for bone health and immune function.
• Vitamin ADE in Serum – Measures vitamins A, D, and E to evaluate nutritional status and potential deficiencies.

Amino Acids & Metabolic Testing

• Amino Acids in Biological Fluids – Used for detecting metabolic disorders and nutritional imbalances.
• Organic Acids in Urine – Helps diagnose metabolic conditions, including inherited metabolic disorders.

Neonatal & Genetic Screening

• Neonatal Screening in DBS (Dried Blood Spot) – A crucial early screening for metabolic and genetic conditions in newborns.
• Phenylketonuria (PKU) Testing (DBS & Serum) – Detects PKU, a rare genetic disorder affecting metabolism.

Neurological & Neurotransmitter Testing

• Biogenic Amines in Urine – Evaluates neurotransmitter levels for conditions related to mood, stress, and nervous system health.
• Neurotransmitters in Urine – Helps assess neurological disorders, mood imbalances, and stress responses.
• VMA / HVA / 5-HIAA in Urine – Used in the diagnosis of neuroendocrine tumors, including pheochromocytoma and neuroblastoma.

Hormonal & Stress Response Markers

• Catecholamines in Urine – Measures stress-related hormones like dopamine, epinephrine, and norepinephrine.
• Metanephrine in Urine – Helps diagnose adrenal gland disorders and monitor stress-related conditions.

Immunosuppressant & Transplant Monitoring

• Immunosuppressant in Whole Blood – Critical for transplant patients to monitor medication levels and avoid rejection risks.

Gut & Metabolic Health

• Gut Dysbiosis Metabolite Markers in Urine – Evaluates microbiome imbalances, aiding in gut health and disease research.

Fatty Acid & Energy Metabolism

• Carnitine / Acylcarnitine in DBS – Assesses energy metabolism and identifies disorders affecting fatty acid oxidation.

Unlock Genetic Insights with Next-Generation Sequencing (NGS) and Bioinformatics

We provide gene sequencing and analysis to support your research and discoveries. Using Next-Generation Sequencing (NGS) technology, we offer solutions to study genetic variations, gene expression, and specific gene targets with precision and speed.

Available Gene Sequencing Options

Whole Genome Sequencing

Whole Genome Sequencing (WGS) offers a complete picture of genetic variations across the entire genome. This service includes:

• Comprehensive Coverage – Sequence all the genetic material to identify mutations, structural variations, and other genetic features.
• Detailed Variant Detection – Accurately identify SNPs, indels, and CNVs for a deeper understanding of genetic differences.

RNA-Seq (Transcriptome Analysis)

RNA-Seq provides an in-depth look at gene expression and how genes are regulated. Key features include:

• Full Transcriptome Profiling – Understand the activity and regulation of genes across different conditions.
• Differential Expression Analysis – Compare gene expression in various samples to discover biomarkers or changes in gene activity.

Custom Sequencing Projects

For focused sequencing of specific genes or regions, we offer:

• Targeted Sequencing – Focus on genes or areas of the genome that are most relevant to your research.
• Exome Sequencing – Sequence the coding regions of the genome to identify mutations in protein-coding genes.
• Amplicon Sequencing – Accurate sequencing of specific areas of the genome with high coverage.

Why Choose This Service?

• Precision & Reliability – Advanced sequencing technology ensures accurate results.
• Customizable – Whether you need full genome data or targeted sequencing, we can meet your exact needs.
• Comprehensive Data Analysis – Along with sequencing, we provide expert bioinformatics support to help you understand the data and draw meaningful conclusions.
• Fast Turnaround – We aim to deliver fast, high-quality results, so you can move your research forward.
• Expert Guidance – Our experienced team is here to assist with every step, from sequencing to analysis.