SDHD Antibody

• Background:

  SDHD Antibody: The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH) . Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.

• NCBI Gene ID:

  9392

• Swiss Prot:

  O14521

• Accession Number:

  NP_002993

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  SDHD Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1287 - EL4 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%), Sheep: (100%), Pig: (80%)

• Buffer:

  SDHD Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SDHD antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.

• Symbol:

  SDHD

• Positive Control 2:

  Cat. No. 17-207 - EL4 Cell Slide

• NCBI Official Name:

  Succinate dehydrogenase complex, subunit D, integral membrane protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Baysal BE, Ferrell RE, Willett-Brozick JE, et al. Mutations in SDHD, a mitochondrial complex II gene, in hereditary paraganglioma. Science 2000; 287:848-51.

• Background Reference 02:

  Saraste M. Oxidative phosphorylation at the fin de siecle. Science 1999; 283:1488-93.

• Background Reference 03:

  Knudson AG. Genetics of human cancer. Annu. Rev. Genet. 1986; 20:231-51.

• Background Reference 04:

  Gimenez-Roqueplo AP, Favier J, Rustin P, et al. The R22X mutation of the SDHD gene in hereditary paraganglioma abolishes the enzymatic activity of Complex II in the mitochondrial respiratory chain and activates the hypoxia pathway. Am. J. Hum. Genet. 2001; 69:1186-97.

• Other Product Names:

  SDHD Antibody: TRAP1, TRAP-1, SDH4, CII-4, CybS

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  4506865

• Physical Properties:

  Liquid