WAS Antibody

• Background :

  The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.

• NCBI Gene ID :

  7454

• Swiss Prot :

  P42768

• Accession Number :

  P42768

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This WAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-234 amino acids from the Central region of human WAS.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Signal Transduction

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  53 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  WAS

• NCBI Official Name :

  Wiskott-Aldrich syndrome protein

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Burns, S.O., et al. Blood 115 (26) :5355-5365 (2010)

• Background Reference 02 :

  Taylor, M.D., et al. Sci Transl Med (37), 37RA44 (2010) :

• Background Reference 03 :

  Rajmohan, R., et al. FEMS Yeast Res. 9 (8) :1226-1235 (2009)

• Background Reference 04 :

  Dovas, A., et al. J. Cell. Sci. 122 (PT 21), 3873-3882 (2009) :

• Other Product Names :

  Wiskott-Aldrich syndrome protein, WASp, WAS, IMD2

• Tested Applications :

  WB

• Physical Properties :

  Liquid