Products for Research Use Only
RegisterLogin

Human complement C3a ELISA Kit PicoKine®

CAT: 0519-EK2145Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0519-EK2145Size:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Complement component 3, often simply called C3, is a protein of the immune system. In humans it is encoded on chromosome 19 by a gene called C3. Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
Description
Human C3a anaphylatoxin ELISA Kit PicoKine® (96 Tests) . Quantitate Human C3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 63 61 62 65 63 3.83 6.1% Sample 2 213 195 232 215 214 11.97 5.6% Sample 3 1095 1045 1002 989 1033 58.87 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 63 61 62 65 63 3.83 6.1% Sample 1 63 61 62 65 63 3.83 6.1% Sample 2 213 195 232 215 214 11.97 5.6% Sample 2 213 195 232 215 214 11.97 5.6% Sample 3 1095 1045 1002 989 1033 58.87 5.7% Sample 3 1095 1045 1002 989 1033 58.87 5.7%
Gene Name
C3
Gene ID
718
UniProt
P01024
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S672-R748
Capture Antibody
Monoclonal antibody from mouse
Detection Antibody
Biotinylated polyclonal antibody from goat
Applications
ELISA
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine
Detection Range
31.2 pg/mL - 2,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 63 213 1095 60 224 1088 Standard deviation 3.28 14.06 50.37 3.06 14.34 38.08 CV (%) 5.2% 6.6% 4.6% 5.1% 6.4% 3.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 63 213 1095 60 224 1088 Standard deviation 3.28 14.06 50.37 3.06 14.34 38.08 CV (%) 5.2% 6.6% 4.6% 5.1% 6.4% 3.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 63 213 1095 60 224 1088 Mean (pg/ml) 63 213 1095 60 224 1088 Standard deviation 3.28 14.06 50.37 3.06 14.34 38.08 Standard deviation 3.28 14.06 50.37 3.06 14.34 38.08 CV (%) 5.2% 6.6% 4.6% 5.1% 6.4% 3.5% CV (%) 5.2% 6.6% 4.6% 5.1% 6.4% 3.5%
Sensitivity
<20 pg/mL
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity
Natural and recombinant human C3
Anticoagulant
Heparin, EDTA or citrate
Sample Data
Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.182 0.295 0.471 0.733 1.108 1.808 3.004 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.182 0.295 0.471 0.733 1.108 1.808 3.004 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.182 0.295 0.471 0.733 1.108 1.808 3.004 O.D. 0.01 0.182 0.295 0.471 0.733 1.108 1.808 3.004
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

Related Products

CatalogName
EK2143Human Complement C2 ELISA Kit PicoKine®
EK0966Human complement C5a ELISA Kit PicoKine®
EK1954Human Complement C1R ELISA Kit PicoKine®
FEK0966Human Complement C5a PicoKine® Quick ELISA Kit
EK0987Mouse Complement C5a ELISA Kit PicoKine®
EK1399Human Complement Factor H/CFH ELISA Kit PicoKine®
EK1954-carrier-freeHuman Complement C1R ELISA Kit PicoKine®, Carrier-free
EK2143-carrier-freeHuman Complement C2 ELISA Kit PicoKine®, Carrier-free
EK1653Human Properdin / Complement Factor P ELISA Kit PicoKine®
FEK0987Mouse Complement C5a PicoKine® Quick ELISA Kit

Alternative Products

CatalogName
MBS1756278-01Human Complement C2 ELISA Kit PicoKine
EK2145Human complement C3a ELISA Kit PicoKine®
MBS177223-01Human complement C5a PicoKine ELISA Kit
EK2143Human Complement C2 ELISA Kit PicoKine®
EK1954Human Complement C1R ELISA Kit PicoKine®
EK0966Human complement C5a ELISA Kit PicoKine®
MBS177646-01Human Complement H/CFH PicoKine ELISA Kit
FEK0966Human Complement C5a PicoKine® Quick ELISA Kit
MBS1756415-01Human Complement C5a PicoKine Quick ELISA Kit
MBS177372-01Mouse Complement C5a PicoKine ELISA Kit

Popular Products