Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)

• Background:

  Beta-D-glucuronidase from E.coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.

• Abbreviation:

  Uida; Beta-Gus

• Synonyms:

  Beta-glucuronidase; GUS; uidA

• UniProt:

  P05804

• Accession Number:

  P05804

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Met1-Gln603

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  69.9 kDa

• Observed Molecular Weight:

  72 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

• Species:

  E.coli