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Human Complement C2 ELISA Kit PicoKine®

CAT: 0519-EK2143Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0519-EK2143Size:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Complement C2 is a protein that in humans is encoded by the C2 gene. Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.
Description
Human Complement C2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human C2 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 785 774 769 782 778 22.55 2.9% Sample 2 1654 1723 1645 1697 1680 43.67 2.6% Sample 3 10235 10156 10619 10665 10306 371.03 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 785 774 769 782 778 22.55 2.9% Sample 1 785 774 769 782 778 22.55 2.9% Sample 2 1654 1723 1645 1697 1680 43.67 2.6% Sample 2 1654 1723 1645 1697 1680 43.67 2.6% Sample 3 10235 10156 10619 10665 10306 371.03 3.6% Sample 3 10235 10156 10619 10665 10306 371.03 3.6%
Gene Name
C2
Gene ID
717
UniProt
P06681
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: A21-L752
Capture Antibody
Monoclonal antibody from mouse
Detection Antibody
Biotinylated polyclonal antibody from goat
Applications
ELISA
Standard
20 ng/Tube
Sample Type
Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine
Detection Range
312 pg/mL - 20,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 785 1654 10235 766 1641 10634 Standard deviation 35.33 71.12 521.99 24.51 59.08 521.07 CV (%) 4.5% 4.3% 5.1% 3.2% 3.6% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 785 1654 10235 766 1641 10634 Standard deviation 35.33 71.12 521.99 24.51 59.08 521.07 CV (%) 4.5% 4.3% 5.1% 3.2% 3.6% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 785 1654 10235 766 1641 10634 Mean (pg/ml) 785 1654 10235 766 1641 10634 Standard deviation 35.33 71.12 521.99 24.51 59.08 521.07 Standard deviation 35.33 71.12 521.99 24.51 59.08 521.07 CV (%) 4.5% 4.3% 5.1% 3.2% 3.6% 4.9% CV (%) 4.5% 4.3% 5.1% 3.2% 3.6% 4.9%
Sensitivity
<20 pg/mL
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity
Natural and recombinant human C2
Anticoagulant
Heparin, EDTA or citrate
Sample Data
Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

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