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Mouse Complement C5a PicoKine® Quick ELISA Kit

CAT: 0519-FEK0987Size: 96 Well/Kit - With Removable StripsDry Ice: NoHazardous: No
CAT#:0519-FEK0987Size:96 Well/Kit - With Removable Strips
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Background
C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
Description
Mouse Complement C5a PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Complement C5a in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 43 45 38 39 41 1.55 3.8% Sample 2 331 235 171 206 235 18.56 7.9% Sample 3 501 531 479 463 493 25.63 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 43 45 38 39 41 1.55 3.8% Sample 1 43 45 38 39 41 1.55 3.8% Sample 2 331 235 171 206 235 18.56 7.9% Sample 2 331 235 171 206 235 18.56 7.9% Sample 3 501 531 479 463 493 25.63 5.2% Sample 3 501 531 479 463 493 25.63 5.2%
Gene Name
C5
Gene ID
15139
UniProt
P06684
Reactivity
Mouse
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: N679-R755
Capture Antibody
Polyclonal antibody from goat
Detection Antibody
Polyclonal antibody from goat
Applications
ELISA
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/mL - 1,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 43 331 501 37 339 575 Standard deviation 1.80 22.50 26.14 2.77 25.42 50.60 CV (%) 4.2% 6.8% 7.9% 6.1% 7.5% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 43 331 501 37 339 575 Standard deviation 1.80 22.50 26.14 2.77 25.42 50.60 CV (%) 4.2% 6.8% 7.9% 6.1% 7.5% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 43 331 501 37 339 575 Mean (pg/ml) 43 331 501 37 339 575 Standard deviation 1.80 22.50 26.14 2.77 25.42 50.60 Standard deviation 1.80 22.50 26.14 2.77 25.42 50.60 CV (%) 4.2% 6.8% 7.9% 6.1% 7.5% 8.8% CV (%) 4.2% 6.8% 7.9% 6.1% 7.5% 8.8%
Sensitivity
<10 pg/mL
Components
Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
Storage Conditions
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Specificity
Natural and recombinant Mouse C5
Anticoagulant
Heparin or EDTA
Sample Data
Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.025 0.181 0.302 0.551 0.986 1.669 2.168 2.310 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.025 0.181 0.302 0.551 0.986 1.669 2.168 2.310 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.025 0.181 0.302 0.551 0.986 1.669 2.168 2.310 O.D. 0.025 0.181 0.302 0.551 0.986 1.669 2.168 2.310
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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