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Human Properdin / Complement Factor P ELISA Kit PicoKine®

CAT: 0519-EK1653Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0519-EK1653Size:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Properdin or Factor P is the only known positive regulator of complement activation that stabilizes thealternative pathway convertases. This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Description
Human Properdin / Complement Factor P ELISA Kit PicoKine® (96 Tests) . Quantitate Human CFP in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 616 576 554 549 573 26.42 4.6% Sample 2 2125 2256 2211 2286 2219 60.73 2.7% Sample 3 9062 9597 10032 10437 9782 510.91 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 616 576 554 549 573 26.42 4.6% Sample 1 616 576 554 549 573 26.42 4.6% Sample 2 2125 2256 2211 2286 2219 60.73 2.7% Sample 2 2125 2256 2211 2286 2219 60.73 2.7% Sample 3 9062 9597 10032 10437 9782 510.91 5.2% Sample 3 9062 9597 10032 10437 9782 510.91 5.2%
Synonyms
Properdin; Complement factor P; CFP; PFC
Gene Name
CFP
Gene ID
5199
UniProt
P27918
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: D28-L469
Capture Antibody
A monoclonal antibody from mouse
Detection Antibody
A biotinylated polyclonal antibody from goat
Applications
ELISA
Field of Research
Microbiology, Protein
Standard
20 ng/Tube
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA) .
Detection Range
312 pg/mL - 20,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 616 2125 9062 602 2285 9683 Standard deviation 28.33 91.37 561.84 30.1 109.68 629.39 CV (%) 4.6% 4.3% 6.2% 5% 4.8% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 616 2125 9062 602 2285 9683 Standard deviation 28.33 91.37 561.84 30.1 109.68 629.39 CV (%) 4.6% 4.3% 6.2% 5% 4.8% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 616 2125 9062 602 2285 9683 Mean (pg/ml) 616 2125 9062 602 2285 9683 Standard deviation 28.33 91.37 561.84 30.1 109.68 629.39 Standard deviation 28.33 91.37 561.84 30.1 109.68 629.39 CV (%) 4.6% 4.3% 6.2% 5% 4.8% 6.5% CV (%) 4.6% 4.3% 6.2% 5% 4.8% 6.5%
Sensitivity
<10 pg/mL
Function
A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity
Natural and recombinant human Properdin
Anticoagulant
Heparin or EDTA
Gene Name Synonym
Complement factor properdin
Subcellular Location
Secreted.
Sample Data
Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.026 0.08 0.119 0.215 0.374 0.716 1.249 2.052 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.026 0.08 0.119 0.215 0.374 0.716 1.249 2.052 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.026 0.08 0.119 0.215 0.374 0.716 1.249 2.052 O.D. 0.026 0.08 0.119 0.215 0.374 0.716 1.249 2.052
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

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