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Mouse Complement C5a ELISA Kit PicoKine®

CAT: 0519-EK0987Size: 96 Well/Kit - With Removable StripsDry Ice: NoHazardous: No
CAT#:0519-EK0987Size:96 Well/Kit - With Removable Strips
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Background
C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
Description
Mouse Complement C5a ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse C5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 40 36 37 37 1.47 3.9% Sample 2 229 230 191 207 214 16.26 7.5% Sample 3 499 528 477 461 491 25.14 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 40 36 37 37 1.47 3.9% Sample 1 38 40 36 37 37 1.47 3.9% Sample 2 229 230 191 207 214 16.26 7.5% Sample 2 229 230 191 207 214 16.26 7.5% Sample 3 499 528 477 461 491 25.14 5.1% Sample 3 499 528 477 461 491 25.14 5.1%
Synonyms
Complement C5; Hemolytic complement; Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain; C5; Hc
Gene Name
C5
Gene ID
15139
UniProt
P06684
Reactivity
Mouse
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: N679-R755
Capture Antibody
Monoclonal antibody from rat
Detection Antibody
Polyclonal antibody from goat
Applications
ELISA
Field of Research
2339, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Immunology, Innate Immunity, Transcription
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
Detection Range
15.6 pg/mL - 1,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 229 499 35 224 532 Standard deviation 1.78 16.94 38.92 2.1 18.81 49.47 CV (%) 4.7% 7.4% 7.8% 6% 8.4% 9.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 229 499 35 224 532 Standard deviation 1.78 16.94 38.92 2.1 18.81 49.47 CV (%) 4.7% 7.4% 7.8% 6% 8.4% 9.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 229 499 35 224 532 Mean (pg/ml) 38 229 499 35 224 532 Standard deviation 1.78 16.94 38.92 2.1 18.81 49.47 Standard deviation 1.78 16.94 38.92 2.1 18.81 49.47 CV (%) 4.7% 7.4% 7.8% 6% 8.4% 9.3% CV (%) 4.7% 7.4% 7.8% 6% 8.4% 9.3%
Sensitivity
<10 pg/mL
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular Weight
188878 MW
Specificity
Natural and recombinant mouse Complement C5a
Anticoagulant
Heparin or EDTA
Gene Name Synonym
Complement C5
Subcellular Location
Secreted.
Sequence Similarities
Contains 1 anaphylatoxin-like domain.
Protein Name
Complement C5
Sample Data
Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.06 0.135 0.214 0.338 0.568 0.893 1.461 1.912 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.06 0.135 0.214 0.338 0.568 0.893 1.461 1.912 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.06 0.135 0.214 0.338 0.568 0.893 1.461 1.912 O.D. 0.06 0.135 0.214 0.338 0.568 0.893 1.461 1.912
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

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