Human Complement Factor H/CFH ELISA Kit PicoKine®

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation') . This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Human Complement Factor H/CFH ELISA Kit PicoKine® (96 Tests) . Quantitate Human CFH in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3203 2994 3170 3050 3104 85.4 2.7% Sample 2 13518 12944 13467 11714 12910 726.54 5.6% Sample 3 53844 53833 53094 52680 53362 497.77 0.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3203 2994 3170 3050 3104 85.4 2.7% Sample 1 3203 2994 3170 3050 3104 85.4 2.7% Sample 2 13518 12944 13467 11714 12910 726.54 5.6% Sample 2 13518 12944 13467 11714 12910 726.54 5.6% Sample 3 53844 53833 53094 52680 53362 497.77 0.9% Sample 3 53844 53833 53094 52680 53362 497.77 0.9%

Complement factor H; H factor 1; CFH; HF, HF1, HF2

CFH

3075

P08603

Human

There is no detectable cross-reactivity with other relevant proteins.

Target protein isolated from human plasma

Monoclonal antibody from mouse

Monoclonal antibody from mouse

Expressed by the liver and secreted in plasma.

ELISA

100 ng/Tube

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

1.56 ng/mL - 100 ng/mL

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3203 13518 53844 3406 12935 57149 Standard deviation 233.81 784.04 3822.92 303.13 905.45 4971.96 CV (%) 7.3% 5.8% 7.1% 8.9% 7% 8.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3203 13518 53844 3406 12935 57149 Standard deviation 233.81 784.04 3822.92 303.13 905.45 4971.96 CV (%) 7.3% 5.8% 7.1% 8.9% 7% 8.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3203 13518 53844 3406 12935 57149 Mean (pg/ml) 3203 13518 53844 3406 12935 57149 Standard deviation 233.81 784.04 3822.92 303.13 905.45 4971.96 Standard deviation 233.81 784.04 3822.92 303.13 905.45 4971.96 CV (%) 7.3% 5.8% 7.1% 8.9% 7% 8.7% CV (%) 7.3% 5.8% 7.1% 8.9% 7% 8.7%

<50 pg/mL

Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b) NBB complex (C5 convertase) in the alternative complement pathway.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

139096 MW

Natural and recombinant human CFH

Heparin or EDTA

Complement factor H

Secreted.

Complement factor H

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.031 0.094 0.157 0.295 0.487 1.032 1.901 2.519 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.031 0.094 0.157 0.295 0.487 1.032 1.901 2.519 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.031 0.094 0.157 0.295 0.487 1.032 1.901 2.519 O.D. 0.031 0.094 0.157 0.295 0.487 1.032 1.901 2.519