Human complement C3a ELISA Kit PicoKine®, Carrier-free

Human complement C3a ELISA Kit PicoKine®, Carrier-free

• Background: Complement component 3, often simply called C3, is a protein of the immune system. In humans it is encoded on chromosome 19 by a gene called C3. Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
• Description: Human C3a anaphylatoxin ELISA Kit PicoKine® (96 Tests). Quantitate Human C3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Gene Name: C3
• Gene ID: 718
• UniProt: P01024
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: S672-R748
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <20 pg/ml
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human C3
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	63	61	62	65	63	3.83	6.1%
  Sample 2	213	195	232	215	214	11.97	5.6%
  Sample 3	1095	1045	1002	989	1033	58.87	5.7%

  *number of samples for each test n=16.
• : heparin, EDTA or citrate