EIF4E2 Antibody

• Background:

  EIF4E2 belongs to the eukaryotic initiation factor 4E family. It recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

• NCBI Gene ID:

  9470

• Swiss Prot:

  O60573

• Accession Number:

  NP_004837

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4E2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF4E2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  27 kDa

• Applications Notes:

  EIF4E2 antibody can be used for detection of EIF4E2 by ELISA at 1:62500. EIF4E2 antibody can be used for detection of EIF4E2 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  EIF4E2

• NCBI Official Name:

  Eukaryotic translation initiation factor 4E family member 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Tee, A.R., FEBS Lett. 564 (1-2), 58-62 (2004) .

• Other Product Names:

  EIF4E2, 4EHP, IF4e, 4E-LP, EIF4EL3

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  4757702

• Physical Properties:

  Liquid