EIF4E Antibody

• Background:

  EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.

• NCBI Gene ID:

  1977

• Swiss Prot:

  P06730

• Accession Number:

  P06730

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This EIF4E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human EIF4E.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine, Mouse, Rabbit, Rat, Xenopus

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  25 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  EIF4E

• NCBI Official Name:

  Eukaryotic translation initiation factor 4E

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rychlik, W., J. Biol. Chem. 262 (22), 10434-10437 (1987)

• Background Reference 02:

  Dorfman, J., Genomics (4), 785-788 (1991)

• Background Reference 03:

  Pelletier, J., Genomics 10 (4), 1079-1082 (1991)

• Background Reference 04:

  Whalen, S.G., J. Biol. Chem. 271 (20), 11831-11837 (1996)

• Other Product Names:

  Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F

• Tested Applications:

  WB, IHC-P

• Protein ID:

  1352435

• Physical Properties:

  Liquid