EIF4G2 Antibody

• Background:

  Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. EIF4G2 shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, EIF4G2 functions as a general repressor of translation by forming translationally inactive complexes.

• NCBI Gene ID:

  1982

• Swiss Prot:

  P78344

• Accession Number:

  AAH14930

• Host:

  Rabbit

• Reactivity:

  Human, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4G2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF4G2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  39 kDa, 98 kDa, 102 kDa

• Applications Notes:

  EIF4G2 antibody can be used for detection of EIF4G2 by ELISA at 1:312500. EIF4G2 antibody can be used for detection of EIF4G2 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  EIF4G2

• NCBI Official Name:

  Eukaryotic translation initiation factor 4 gamma, 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  NIH MGC Project, (2001) Direct Submission.

• Other Product Names:

  EIF4G2, P97, AAG1, DAP5, NAT1

• Tested Applications:

  ELISA, WB

• Protein ID:

  34782924

• Physical Properties:

  Liquid