EIF4A2 Antibody

• Background:

  EIF4A2 contains 1 helicase C-terminal domain and 1 helicase ATP-binding domain. It belongs to the DEAD box helicase family. eIF4A subfamily. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5' untranslated region of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

• NCBI Gene ID:

  1974

• Swiss Prot:

  Q14240

• Accession Number:

  NP_001958

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4A2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF4A2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  45 kDa

• Applications Notes:

  EIF4A2 antibody can be used for detection of EIF4A2 by ELISA at 1:312500. EIF4A2 antibody can be used for detection of EIF4A2 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  EIF4A2

• NCBI Official Name:

  Eukaryotic translation initiation factor 4A2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sarkar, M., (2005) FEBS Lett. 579 (16), 3449-3460.

• Other Product Names:

  EIF4A2, DDX2B, EIF4A, EIF4F, BM-010, eIF4A-II, eIF-4A-II

• Tested Applications:

  ELISA, WB

• Protein ID:

  83700235

• Physical Properties:

  Liquid