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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide located in solubilized part of the protein, derived from available di and monocot PsbS sequences, including Arabidopsis thaliana (At1g44575)
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS03 032 | anti-PsbS hen antibodyPSII available antibodies against Photosystem II proteinsPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1 : 2000 with standar ECL (WB)
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Molecular weight expected аpparent
28 | 22 kDa for Arabidopsis thaliana
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Verified reactivity
Arabidopsis thaliana, Flaveria bidentis, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Picea abies, Ricinus communis, Spinacia oleracea, Zea mays
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Possible reactivity
dicots including: Cucumis sativus, Medicago truncatula, Solanum lycopersicum, Vitis vinifera, monocots including: Oryza sativa, trees: Picea sitchensis, Pinus radiata, Pinus taeda, Populus balsamifera, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
to be added when available
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References
Poudyal et al. (2016). Production of superoxide from photosystem II-light harvesting complex II supercomplex in STN8 kinase knock-out rice mutants under photoinhibitory illumination. J Photochem Photobiol B. 2016 Sep;162:240-7. doi: 10.1016/j.jphotobiol.2016.06.050. Ishikawa et al. (2016). NDH-Mediated Cyclic Electron Flow Around Photosystem I is Crucial for C4 Photosynthesis. Plant Cell Physiol. 2016 Aug 6. pii: pcw127. [Epub ahead of print] Pavlovic et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009. Karlsson et al. (2015). The Arabidopsis thylakoid transporter PHT4;1 influences phosphate availability for ATP synthesis and plant growth. Plant J. 2015 Aug 8. doi: 10.1111/tpj.12962. Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479. Belgio et al. (2015). Light harvesting superstructures of green plant chloroplasts lacking photosystems. Plant Cell Environ. 2015 Mar 4. doi: 10.1111/pce.12528. Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16.Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16.Albus et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.
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Scientific context
The 22 kDa PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence. Synonymes: NPQ4 (NONPHOTOCHEMICAL QUENCHING).
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Notes
This product can be sold containing proclin if requested.
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Protein number
Q9XF91
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TAIR number
AT1G44575
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Description
The kilo Daltons subunit weight of PsbS, 22 Lhc-like PSII protein compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
