-
Available ordering format
Lyophilized in PBS pH 7.4
-
Immunogen
native, purified 23 kDa protein from Spinacia oleracea
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Total IgG
-
How to reconstitute
For reconstitution add 200 µl of sterile water.
-
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
western blot (WB)
-
Connected products
AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSIIAS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSIIAS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSIIAS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSIIAS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSIIPlant protein extraction buffer Secondary antibodies
-
Recommended dilutions for use
1:2000 - 1: 5000 with standard ECL (WB)
-
Molecular weight expected аpparent
28 | 23 kDa
-
Verified reactivity
Arabidopsis thaliana , Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Chlamydomonas reinhardti
-
Possible reactivity
dicots including: Pisum sativum, Solanum lycopersicum, monocots including: Oryza sativa, trees including: Populus balsamifera, Pinus monticola
-
No reactivity
Synechococcus sp. PCC 7942
-
Supplementary information
Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).
-
References
Wang et al. (2008). Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana. Nat Chem Biol. 4: 557-563.
-
Scientific context
PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
-
Notes
None
-
Protein number
Refer to NCBI
-
TAIR number
Refer to NCBI
-
-
Description
The kilo Daltons subunit weight of PsbP, 23 protein of the oxygen evolving complex (OEC) of PSII compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
