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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide chosen from known sequences of PsbC including Arabidopsis thaliana PsbC, UniProt: P56778, TAIR: AtCg00280
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS10 111S | CP43' | IsiA homolog of plant CP43 protein standard PSII antibody collectionPlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1:3 000 with ECL (WB)
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Molecular weight expected аpparent
45 | 43 kDa
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Verified reactivity
Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Oryza sativa, Panax ginseng, Phaseolus vulgaris, Synechocystis sp. PCC6803, Vigna radiate
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Possible reactivity
Asimina parviflora, Borago officinalis, Carthamus persicus, Casimirella guaranitica , Catalpa bungei, Calatola mollis, Citron x limon, Cunninghamia lanceolata, Deeringothamnus rugelii, Gonystylus bancanus, Ipomopsis aggregata, Leretia cordata, Lobatiriccardia lobata, Myricaria germanica , Nostoc sp. PCC7120, Nannochloropsis sp., Natsiatum herpeticum, Nothapodytes montana , Nerium oleander, Ottoschulzia rhodoxylon, Oxandra lanceolata,Solanum tuberosum, Oryza sativa, Panax quinquefolius, Prosopidastrum angusticarpum, Prosopis glandulosa, Rollinia mucosa, Rosmarinus officinalis, Saxifraga rivularis, Spinacia oleracea, Zelkova serrata, Zinnia violacea, Length=, Vachellia caven, Vitis vinifera, Xerocladia viridiramis
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This product can be sold containing ProClin if requestedIn C4 plants like Echinochloa crus-galli and Zea mays antibody detects 2 bands.
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References
Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. Mazur et al. BMC Plant Biology (2016) 16:191. Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015. Chen et al. (2016). Expression of holo-proteorhodopsin in Synechocystis sp. PCC 6803. Metab Eng. 2016 Feb 8;35:83-94. doi: 10.1016/j.ymben.2016.02.001. Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29. Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675. Calderon et al. (2013). A Conserved Rubredoxin is Necessary for Photosystem II Accumulation in Diverse Oxygenic Photoautotrophs. J Biol Chem. July 30. (reference for reactivity in Chlamydomonas reinhardtii)Sakuraba et al. (2013). The green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions. Plant J.Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.
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Scientific context
PsbC (CP43) acts as an antenna to the PSII core and its presence seem to be also necessary for maintaining water splitting activity. This protein is more weakly associated with the PSII reaction centre and can be removed from the isolated core.
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Notes
None
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Protein number
P56778
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TAIR number
ATCG00280
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