PsbP, 23 kDa protein of the oxygen evolving complex (OEC) of PSII

• Catalog number
  AS06 167
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated synthetic peptide derived from PsbP protein of Arabidopsis thaliana Q42029, At1g06680
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 100 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSIIAS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSIIAS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSIIAS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSIIPlant protein extraction buffer Secondary antibodies
• Recommended dilutions for use
  1 : 2000 with standard ECL (WB)
• Molecular weight expected аpparent
  28 | 23 kDa (Arabidopsis thaliana)
• Verified reactivity
  Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Picea abies, Pisum sativum, Physcomitrella patens
• Possible reactivity
  dicots including: Solanum tuberosum, monocots including: Oryza sativa, Triticum aestivum, trees: Picea sitchenisis, Populus balsamifera, moss:
• No reactivity
  Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942
• Supplementary information
  If you work with Chlamydomonas reinhardii, please use following PsbP antibody: AS06 142-23
• References
  Pavlovic et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009. Albanese et al. (2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.Grassl et al. (2012). Early events in plastid protein degradation in stay-green Arabidopsis reveal differential regulation beyond the retention of LHCII and chlorophyll. J. Proteome Res. October 2.Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
• Scientific context
  PsbP - 23 kDa extrinsic protein of photosystem II (PSII). Processing of the protein results in a protein with molecular mass of around 20 kDa. PsbP is required to optimize water splitting process in PSII, by probaböy by optimisation of calcium and Cl- levels. The protein is found in higher plants and algae but is not conserved in cyanobacteria. Synonymes:Oxygen-evolving enhancer protein 2-1, chloroplastic, OEE2, 23 kDa subunit of oxygen evolving system of photosystem II, OEC 23 kDa subunit, OEC23, 23 kDa thylakoid membrane protein
• Notes
  None
• Protein number
  Q42029
• TAIR number
  AT1G06680
• Hyperlink
• Description
  The kilo Daltons subunit weight of PsbP, 23 protein of the oxygen evolving complex (OEC) of PSII compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein

• Gene target
  PsbP   protein   the   oxygen   evolving   complex   OEC   PSII  
• Short name
  PsbP, 23 protein of the oxygen evolving complex (OEC) of PSII
• Alternative name
  PsbP, 23 kiloDalton protein on the oxygen evolving aggregate (OEC) on PSII

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