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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
Native purified 33 kDa protein from Spinacia oleracea UniProt P12359
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Western Blot (WB)
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Connected products
AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSIIAS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSIIAS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSIIAS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSIIPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1:2000-1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent
35 | 33 kDa
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Verified reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sp. DT, Festuca arundinacea cv. Kord, Haematococcus pluvialis, Hordeum spontaneum, Hordeum vulgare, Picea abies, Pinus banksiana, Spinacia oleracea, Solanum tuberosum cultivar Taedong Valley, Synechococcus sp. PCC 7942, Thellungiella salsuginea
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Possible reactivity
dicots including: Nicotiana tabacum, Solanum lycopersicum, trees: Populus trichocarpa
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No reactivity
No confirmed exceptions from predicted reactivity known at the moment
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Supplementary information
This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).
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References
Zhang et al. (2016). A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis. Biotechnol Bioeng. 2016 Oct;113(10):2088-99. doi: 10.1002/bit.25976. Epub 2016 Mar 28.Pavlovic et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.Jedmowski et al. (2014). Comparative analysis of drought stress effects on photosynthesis of Eurasian and North African genotypes of wild barley. Photosynthetica, September 2014.Wiciarz et al. (2014).Enhanced chloroplastic generation of H2 O2 in stress-resistant Thellungiella salsuginea in comparison to Arabidopsis thaliana. Physiol Plant. 2014 Jun 24. doi: 10.1111/ppl.12248.
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Scientific context
PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
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Notes
Total IgG fraction has been purified by 40% ammonium sulpgate precipitation followed by DEAE cellulose chromatography
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Description
The kilo Daltons subunit weight of PsbO, 33 of the oxygen evolving complex (OEC) of PSII compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
