-
Available ordering format
Inquire
-
Immunogen
to be determined
-
Raised in
N/A
-
Clonality
N/A
-
Clone
N/A
-
Purification
Affinity purified
-
How to reconstitute
For reconstitution add 225 µl of milliQ water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
-
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
Western blot (WB)
-
Connected products
collection of other protein standardsAS06 146 | anti-PsbD | D2 protein of PSII antibodiescollection of other global antibodies collection of antibodies to PSII proteins
-
Recommended dilutions for use
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a PsbD signal in this range.Positive control: a 2μl load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
-
Molecular weight expected аpparent
in most gel systems PsbD migrates around 28-30 kDa
-
Verified reactivity
to be determined
-
Possible reactivity
to be determined
-
No reactivity
to be determined
-
Supplementary information
Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.25 pmoles/ulProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
-
References
Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
-
Scientific context
D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.This product is a recombinant protein standard, source Synechocystis strain PCC 6803.
-
Notes
The PsbD protein standard can be used in combination with global anti-PsbD antibodies to quantitate PsbD from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PsbD protein.Quantitative western blot: detailed method description, video tutorial
-
Protein number
Refer to NCBI
-
TAIR number
Refer to NCBI
-
-
Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
-
Group
positif
