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Available ordering format
Lyophilized
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Immunogen
KLH-comjugated synthetic peptide derived from the C-terminal of known PsbD sequences including Arabidopsis pumila A4QJS8 , Hordeum vulgare P11849, Chlamydomonas reinhardtii P06007, Synechococcus sp. PCC 7002 P20898
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water to each tube.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS09 146S | PsbD | D2 protein of PSII protein standard for quantitation and as a positive controlAS06 146PRE | PsbD | D2 protein of PSII, pre-immune serumAS05 084 | anti-PsbA (D1) protein of PSII, C-terminalcollection of antibodies to PSII proteinsrecommended secondary antibodyPlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent
39.4 | 28-30 kDa
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Verified reactivity
Arabidopsis thaliana, Horderum vulgare, Chlamydomonas reinhardtii, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Trifolium pratense, Ulva prolifera, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Anabaena 7120, Ditylum brightwellii, Thalassiosira guillardii, Thalassiosira pseudonana
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Possible reactivity
Glycine max, Panax ginseng, Pinus thunbergii, Physcomitrella patens, Populus trichocarp, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Vitis vinifera, Zea mays
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
There is a confirmed cross-reaction with TLA1 protein in Chlamydomonas reinhardtii.For samples with a very low PSII content theremight be detection problems independent of the antibody. PSII proteins can vary in level depending upon liquid culture conditions. When the cells are in a stationary phase PSII content can drop to a very low level.
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References
Correa-Galvis et al. (2016). PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nature Plants 2, Article number: 15225 (2016) doi:10.1038/nplants.2015.225 Huang et al. (2015). Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis. Volume 236, July 2015, Pages 185-194. Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741. Malnoë et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21.
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Scientific context
D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.
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Notes
The peptide used to elicit this antibody has a perfect conservation across all full-length PsbD sequences from higher plants, lower plants, cyanobacteria and unicellular algae except: -minor substitutions in some Prochlorococcus & Dinoflagellate sequences. The antibody should still work against these taxa, but it has not been tested yet. This antibody does not detect PsbA protein (D1).
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Protein number
A4QJS8 , P11849 , P06007 , P20898
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TAIR number
ATCG00270
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