-
Available ordering format
Lyophilized
-
Immunogen
N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 At5g66570 and 2 proteins At3g50820
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Serum
-
How to reconstitute
For reconstitution add 100 µl of sterile water.
-
Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
-
Connected products
AS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSIIAS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSIIAS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSIIAS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSIIPlant protein extraction buffer Secondary antibodies
-
Recommended dilutions for use
1: 1000 with ECL (WB)
-
Molecular weight expected аpparent
33 kDa
-
Verified reactivity
Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Pisum sativum, Sinapsis alba, Zea mays
-
Possible reactivity
dicots including Brassica oleracea, Pisum sativum, Vitis vinifera, Populus tremula, Picea sitchensis
-
No reactivity
Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942
-
Supplementary information
Good signal is obtained with this antibody with a load from 0.5 chlorophyll µg/well.
-
References
Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. Mazur et al. BMC Plant Biology (2016) 16:191.Albanese et al.(2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.Hu et al. (2015). Site-specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis. Plant Physiol. 2015 Feb 19. pii: pp.00026.2015.Casanova-Sáez et al. (2014). Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development. J Exp Bot. 2014 Mar 24. Albus et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.
-
Scientific context
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.
-
Notes
Loading based on 50-100 ng of chlorophyll is enough to obtain good signal with this antibody
-
Protein number
P23321 , Q9S841
-
TAIR number
AT5G66570
-
-
Description
The kilo Daltons subunit weight of PsbO, 33 of the oxygen evolving complex (OEC) of PSII compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
