HP1 gamma Recombinant Rabbit Monoclonal Antibody [JU81-36]

HP1 gamma Recombinant Rabbit Monoclonal Antibody [JU81-36]

• Description: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of Histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated.
• Product Name Alternative: CBX 3 antibody CBX3 antibody CBX3_HUMAN antibody Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody Chromobox homolog 3 antibody Chromobox protein homolog 3 antibody GAMMA antibody HECH antibody Heterochromatin like protein 1 antibody Heterochromatin protein 1 homolog gamma antibody Heterochromatin protein HP1 gamma antibody HP1 gamma antibody HP1 gamma homolog antibody HP1Hs gamma antibody Modifier 2 protein antibody
• Abbreviation: HP1 gamma, CBX3
• CAS Number: 9000-83-3
• Swiss Prot: Q13185 Human, P23198 Mouse, Q5RJK5 Rat
• Cellular Locus: Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human HP1 gamma aa 38-87 / 183.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, FC, IP
• Positive Control: PC-12 cell lysate, MCF-7 cell lysate, mouse colon tissue lysates, rat testis tissue, human liver carcinoma tissue, human tonsil tissue, human placenta tissue, mouse colon tissue, mouse testis tissue, Hela.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: 1:10-1:50
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 21 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes