Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06]

Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06]

• Description: Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.
• Product Name Alternative: EIF 2 alpha antibody EIF 2 antibody EIF 2A antibody EIF 2alpha antibody eIF-2-alpha antibody eIF-2A antibody EIF-2alpha antibody EIF2 alpha antibody EIF2 antibody EIF2A antibody EIF2S1 antibody Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody Eukaryotic translation initiation factor 2 subunit 1 alpha antibody Eukaryotic translation initiation factor 2 subunit 1 antibody Eukaryotic translation initiation factor 2 subunit alpha antibody IF2A_HUMAN antibody
• Abbreviation: EIF2A, CDA02, MSTP004, MSTP089
• CAS Number: 9000-83-3
• Swiss Prot: P05198 Human, Q6ZWX6 Mouse, P68101 Rat
• Cellular Locus: Stress granule, Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser51 of Human eIF-2a.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
• Positive Control: HeLa treated with 50nM Calyculin A for 3 hours whole cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate, C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate, THP-1 cell lysate, C2C12 cell lysate, mouse spleen tissue, rat spleen tissue, human liver tissue, human pancreas tissue, mouse brain tissue, mouse placenta tissue, mouse pancreas tissue, human prostate carcinoma tissue, human breast carcinoma tissue, human colon carcinoma tissue, Hela.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:500 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 36 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes