Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882]

Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882]

• Description: Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
• Product Name Alternative: Drosophila, homolog of, MADR2 antibody hMAD-2 antibody HsMAD2 antibody JV18 antibody JV18-1 antibody JV181 antibody MAD antibody MAD homolog 2 antibody MAD Related Protein 2 antibody Mad-related protein 2 antibody MADH2 antibody MADR2 antibody MGC22139 antibody MGC34440 antibody Mother against DPP homolog 2 antibody Mothers against decapentaplegic homolog 2 antibody Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody Mothers against DPP homolog 2 antibody OTTHUMP00000163489 antibody Sma and Mad related protein 2 antibody Sma- and Mad-related protein 2 MAD antibody SMAD 2 antibody SMAD family member 2 antibody SMAD, mothers against DPP homolog 2 antibody SMAD2 antibody SMAD2_HUMAN antibody
• Abbreviation: MAD homolog 2, Mothers against DPP homolog 2, hMAD-2, SMAD 2, Smad2, hSMAD2
• CAS Number: 9000-83-3
• Swiss Prot: Q15796 Human, Q62432 Mouse, O70436 Rat
• Cellular Locus: Nucleus, Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser255 of Human Smad2 aa 229-272 / 467.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IP
• Positive Control: HeLa cell lysate, HeLa treated with 200nM Calyculin A for 1 hour cell lysate, RAW264.7 cell lysate, mouse uterus tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 52 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes