EIF2S1 Rabbit Polyclonal Antibody

EIF2S1 Rabbit Polyclonal Antibody

• Description: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming.
• Product Name Alternative: EIF 2 alpha antibody EIF 2 antibody EIF 2A antibody EIF 2alpha antibody eIF-2-alpha antibody eIF-2A antibody EIF-2alpha antibody EIF2 alpha antibody EIF2 antibody EIF2A antibody EIF2S1 antibody Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody Eukaryotic translation initiation factor 2 subunit 1 alpha antibody Eukaryotic translation initiation factor 2 subunit 1 antibody Eukaryotic translation initiation factor 2 subunit alpha antibody IF2A_HUMAN antibody
• Abbreviation: EIF2S1
• CAS Number: 9007-83-4
• Swiss Prot: P05198 Human, Q6ZWX6 Mouse, P68101 Rat
• Cellular Locus: Stress granule.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within human EIF2S1 aa 116-315/315.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Rabbit polyclonal Antibody
• Applications: WB, IHC-P, FC, IF-Cell
• Positive Control: HepG2 cell lysate, A431 cell lysate, Raji cell lysate, Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse colon tissue lysate, rat spleen tissue lysate, human kidney tissue, Hela, A375, NCI-H441.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1,000 ;IHC-P: 1:1,900 ;FC: 1:500-1:1,000 ;IF-Cell: 1:100-1:200
• Purity: Immunogen affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 36 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: No