Phospho-EGFR (Y1068) Recombinant Rabbit Monoclonal Antibody [SD2055]

Phospho-EGFR (Y1068) Recombinant Rabbit Monoclonal Antibody [SD2055]

• Description: Epidermal growth factor mediates its effects on cell growth through its inter-action with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGF alpha to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Once activated, EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2 through direct interactions with Tyr 1068 and Tyr 1086 and through indirect interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptor.
• Product Name Alternative: Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody Cell growth inhibiting protein 40 antibody Cell proliferation inducing protein 61 antibody EGF R antibody EGFR antibody EGFR_HUMAN antibody Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody Epidermal growth factor receptor antibody erb-b2 receptor tyrosine kinase 1 antibody ERBB antibody ERBB1 antibody Errp antibody HER1 antibody mENA antibody NISBD2 antibody Oncogen ERBB antibody PIG61 antibody Proto-oncogene c-ErbB-1 antibody Receptor tyrosine protein kinase ErbB 1 antibody Receptor tyrosine-protein kinase ErbB-1 antibody SA7 antibody Species antigen 7 antibody Urogastrone antibody v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody wa2 antibody Wa5 antibody
• Abbreviation: EGFR
• CAS Number: 9000-83-3
• Swiss Prot: P00533 Human
• Cellular Locus: Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Nucleus membrane, Endosome, Nucleus, Secreted.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGFR.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IP, FC
• Positive Control: HeLa cell lysate, HeLa treated with 100ng/mL EGF for 30 minutes cell lysate, A431, Hela, MCF-7.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 134 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes