Complement Factor D / CFD Recombinant Protein

• Background:

  Complement factor D (CFD) is also known as Adipsin, C3 convertase activator, Properdin factor D (PFD), which contains one peptidase S1 domain and belongs to the peptidase S1 family. CFD / Adipsin cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. CFD / Adipsin is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement factor D deficiency which predisposes to invasive meningococcal disease.

• NCBI Gene ID:

  1675

• Swiss Prot:

  P00746

• Accession Number:

  NP_001919.2

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  50 mM Tris, 150 mM NaCl, pH8.0

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  26.3 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.3 kDa. The protein migrates as 28 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Complement factor D

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  White R.T., et al., 1992, J. Biol. Chem. 267:9210-9213.

• Background Reference 02:

  Johnson D.M.A., et al., 1984, FEBS Lett. 166:347-351.

• Background Reference 03:

  Sprong T., et al., 2006, Blood 107:4865-4870.

• Other Product Names:

  CFD, Adipsin, PFD, DF, Complement factor D

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3