RecombinantSCF, Mouse

CAT: 0384-BK0345-01Size: 10 µgDry Ice: NoHazardous: No
CAT#:0384-BK0345-01Size:10 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant mouse Stem Cell Factor (rmSCF) produced in P. pastoris is a single glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rmSCF has a molecular mass of 18.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 < 10.0 ng/ml, measured by a cell proliferation assay using human TF-1 cells, corresponding to a specific activity of > 1.0x 10ˆ5 units/mg.
Reconstitution
Reconstituted in ddH2O at 100 μg/ml.
Molecular Weight
18.4kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant mouse Stem Cell Factor (rmSCF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmSCF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against 50mM Tris, pH8.0.
Host or Source
P. pastoris
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
MKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVA

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