RecombinantFGF-basic, Mouse

CAT: 0384-BK0057-01Size: 10 µgDry Ice: NoHazardous: No
CAT#:0384-BK0057-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibroblast Growth Factor-basic (FGF-basic), also known as HBGF-2, is a non-glycosylated heparin-binding growth factor that belongs to the FGF family. FGF-basic is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. FGF-basic signals through FGFR1, 2, 3 and 4 that plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration.Recombinant mouse Fibroblast Growth Factor-basic (rmFGF-basic) produced in E.coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rmFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 < 0.5 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 2.0x 10ˆ6 units/mg.
Reconstitution
Reconstituted in ddH2O at 100 μg/ml.
Molecular Weight
16.4 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant mouse Fibroblast Growth Factor-basic (rmFGF-basic) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-basic should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS

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