RecombinantShh, Mouse

• Description:

  Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are threerelatedHh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) . They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical) . Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant mouseSonic Hedgehog (rmShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 176 amino acids. A fully biologically active molecule, rmShh, is obtained by proprietary chromatographic techniques at GenScript with a molecular mass of 19.8kDa analyzed by reducing SDS-PAGE.

• Endotoxin:

  <0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% by SDS-PAGE and HPLC analyses.

• Bioactivity:

  ED50<1.0μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of>1.0x 10ˆ3units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/ml.

• Molecular Weight:

  19.8kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant mouseSonic Hedgehog (rmShh) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmShhshould be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  IVI GPGRGFGKRR HPKKLTPLAY KQFIPNVAEK TLGASGRYEG KITRNSERFK ELTPNYNPDI IFKDEENTGA DRLMTQRCKD KLNALAISVM NQWPGVKLRV TEGWDEDGHH SEESLHYEGR AVDITTSDRD RSKYGMLARL AVEAGFDWVY YESKAHIHCS VKAENSVAAK SGG