RecombinantMIF, Mouse

CAT: 0384-BK0134-01Size: 10 µgDry Ice: NoHazardous: No
CAT#:0384-BK0134-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Macrophage Migration Inhibitory Factor (MIF) is a pleiotropic cytokine, existing as a homotrimer in vivo. MIF was originally identified as a T cell derived factor responsible for the inhibition of macrophage migration. However, recently MIF has received much more attention because of its possible roles in angiogenesis and cancer development. MIF is over-expressed in various cancers, including pancreatic, breast, colon, brain, prostate, skin, and lung. The intratumoral expression MIF is strongly correlated with angiogenic growth factor expression, such as the expression of Interleukin 8 (IL-8) and Vascular Endothelial Growth Factor (VEGF), and with risk of recurrence after resection. Recombinant mouse Macrophage Migration Inhibitory Factor (rmMIF) produced in E.coli is a single non-glycosylated polypeptide chain containing 115 amino acids. rmMIF has a molecular mass of 12.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE and HPLC.
Bioactivity
Bioassay data are not available.
Reconstitution
Reconstituted in ddH2O at 100 μg/mL.
Molecular Weight
12.5 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant mouse Macrophage Migration Inhibitory Factor (rmMIF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmMIF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
MPMFIVNTN VPRASVPEGF LSELTQQLAQ ATGKPAQYIA VHVVPDQLMT FSGTNDPCAL CSLHSIGKIG GAQNRNYSKL LCGLLSDRLH ISPDRVYINY YDMNAANVGW NGSTFA

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