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RecombinantMCP-5/CCL12, Mouse

CAT: 0384-BK0266-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0384-BK0266-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Monocyte Chemotactic Protein-5 (MCP-5), also known as Chemokine (C-C motif) ligand 12 (CCL12), is a small cytokine belonging to the CC chemokine family that has been characterized in mice. Due to its similarity with human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. MCP-5 specifically attracts eosinophils, monocytes and lymphocytes.It can signal through the CCR2 receptor.Recombinant Mouse MCP-5/CCL1 produced in CHO cells is a polypeptide chain containing 82 amino acids. A fully biologically active molecule, rmMCP-5/CCL12 has a molecular mass of 11 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 98% as analyzed by SDS-PAGE.
Bioactivity
The EC50 value of mouse MCP-5/CCL12 on Caˆ2+ mobilization assay in CHO-K1/G15/mCCR2 cells (human G15 and mouse CCR2 stably expressed in CHO-K1 cells) is less than 0.5 μg/ml.
Reconstitution
Reconstituted in ddH2O or PBS at 100 μg/ml.
Molecular Weight
9.3-11 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant Mouse MCP-5/CCL12 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse MCP-5/CCL12 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
CHO
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWV KNSINHLDKTSQTFILEPSCLG

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