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RecombinantMCP-5/CCL12, Mouse

CAT: 0013-GTR18990275-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18990275-01Size:1 mg
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Description
Monocyte Chemotactic Protein-5 (MCP-5), also known as Chemokine (C-C motif) ligand 12 (CCL12), is a small cytokine belonging to the CC chemokine family that has been characterized in mice. Due to its similarity with human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. MCP-5 specifically attracts eosinophils, monocytes and lymphocytes.It can signal through the CCR2 receptor.Recombinant Mouse MCP-5/CCL1 produced in CHO cells is a polypeptide chain containing 82 amino acids. A fully biologically active molecule, rmMCP-5/CCL12 has a molecular mass of 11 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Product Name Alternative
MCP-5, Monocyte Chemotactic Protein-5, CCL12
Source
CHO
Field of Research
Immunology & Inflammation
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 98% as analyzed by SDS-PAGE.
Bioactivity
The EC50 value of mouse MCP-5/CCL12 on Caˆ2+ mobilization assay in CHO-K1/G15/mCCR2 cells (human G15 and mouse CCR2 stably expressed in CHO-K1 cells) is less than 0.5 μg/ml.
Form
Lyophilized after extensive dialysis against PBS.
Molecular Weight
9.3-11 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant Mouse MCP-5°CCL12 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse MCP-5°CCL12 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O or PBS at 100 μg/ml.
Preservative
Lyophilized after extensive dialysis against PBS.
AA Sequence
GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWV KNSINHLDKTSQTFILEPSCLG

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