Recombinant Mouse MCP-5 (CCL12)

• Description:

  MCP-5 (CCL12) is a chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. It is also a potent active monocyte chemokine that signals through CCR2. It is Involved in allergic inflammation and the host response to pathogens. Also, it may play a pivotal role during early stages of allergic lung inflammation.

• CAS Number:

  9000-83-3

• Gene Name:

  CCL12

• Gene Aliases:

  MCP-1-related chemokine, Monocyte chemotactic protein 5, MCP5, Small-inducible cytokine A12, C-C motif chemokine 12, Mcp5, Scya12
  Recombinant Mouse Monocyte Chemoattractant Protein-5 (CCL12)

• Gene ID:

  20293

• Accession Number:

  Q62401

• Organism:

  Mouse

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  Determined by its ability to chemoattract human monocytes using a concentration range of 20-40 ng/mL.

• Molecular Weight:

  10

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Recombinant MCP-5 was lyophilized from a 0.2 μm filtered 20 mM PB solution pH 7.5, 100 mM NaCl.