RecombinantMIP-2/CXCL2, Mouse

CAT: 0384-BK0138-01Size: 5 μgDry Ice: NoHazardous: No
CAT#:0384-BK0138-01Size:5 μg
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Description
Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2) . CXCL2 is 90% identical in amino acid sequence to a related chemokine, CXCL1. CXCL2 is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. CXCL2 can signal through the CXCR2 receptor.Recombinant mouse MIP-2/CXCL2 produced in E.coli is a single non-glycosylated polypeptide chain containing 73 amino acids. A fully biologically active molecule, rmMIP-2/CXCL2 has a molecular mass of 8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE.
Bioactivity
The EC50 value of mouse MIP-2/CXCL2 on Caˆ2+ mobilization assay in CHO-K1/Gα15/mCXCR2 cells (human Gα15 and mouse CXCR2 stably expressed in CHO-K1 cells) is less than 1 ng/ml.
Reconstitution
Reconstituted in ddH2O or PBS at 100 μg/ml.
Molecular Weight
8.0 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant mouse MIP-2/CXCL2 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, mouse MIP-2/CXCL2 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
GA VVASELRCQC LKTLPRVDFK NIQSLSVTPP GPHCAQTEVI ATLKGGQKVC LDPEAPLVQK IIQKILNKGK AN

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