RecombinantMIP-3α/CCL20, Human

• Description:

  Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC) or Macrophage Inflammatory Protein-3 (MIP3A) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus. It can signal through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors.Recombinant Human MIP-3α/CCL20 produced in E.coliis a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMIP-3α/CCL20has a molecular mass of 8.0 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% as analyzed by SDS-PAGE.

• Bioactivity:

  The EC50 value of humanMIP-3 alpha/CCL20 on Caˆ2+ mobilization assay in CHO-K1/G15/hCCR6 cells (human Ga15 and human CCR6 stably expressed in CHO-K1 cells) is less than 200 ng/ml.

• Reconstitution:

  Reconstituted in ddH2O or PBS at 100 μg/ml.

• Molecular Weight:

  8.0 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant Human MIP- 3α/CCL20 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant human MIP-3α/CCL20 should be stable up to 1 week at 4°C or up to 2 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  2424

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  ASN FDCCLGYTDR ILHPKFIVGF TRQLANEGCD INAIIFHTKK KLSVCANPKQ TWVKYIVRLL SKKVKNM