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RecombinantIL-5, Mouse

CAT: 0384-BK0107-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0384-BK0107-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interleukin-5 (rmIL-5), produced by mast cells, T cells and eosinophils, is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF) . It can increase production and mobilization of eosinophils and CD34+ progenitors from the bone marrow. IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release.Recombinant mouse Interleukin-5 (rmIL-5) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 113 amino acids each. A fully biologically active molecule, rmIL-5 has a molecular mass of 26.2kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 < 2.0 ng/ml, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 5.0x 10ˆ5 units/mg.
Reconstitution
Reconstituted in ddH2O at 100 μg/ml.
Molecular Weight
26.2kDa, observed by non-reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant mouse Interleukin-5 (rmIL-5) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-5 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
MEIPMSTVV KETLTQLSAH RALLTSNETM RLPVPTHKNH QLCIGEIFQG LDILKNQTVR GGTVEMLFQN LSLIKKYIDR QKEKCGEERR RTRQFLDYLQ EFLGVMSTEW AMEG

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