RecombinantIL-5, Human

• Description:

  Interleukin-5 (IL-5), produced by mast cells, T cells and eosinophils, is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF) . It can increase production and mobilization of eosinophils and CD34+ progenitors from the bone marrow. IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release.Recombinant Human Interleukin-5 (rhIL-5) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 116 amino acids each. A fully biologically active molecule, rhIL-5 has a molecular mass of 26.5 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% by SDS-PAGE.

• Bioactivity:

  ED50 < 1.0 ng/ml, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 1.0x 10ˆ6 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/ml.

• Molecular Weight:

  26.5 kDa, observed by non-reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant Human Interleukin-5 (rhIL-5) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-5 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against 25mM Tris, pH8.0.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  MIPTEIPTS ALVKETLALL STHRTLLIAN ETLRIPVPVH KNHQLCTEEI FQGIGTLESQ TVQGGTVERL FKNLSLIKKY IDGQKKKCGE ERRRVNQFLD YLQEFLGVMN TEWIIES