RecombinantFGF-basic, Bovine

• Description:

  Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.Recombinant bovine Fibroblast Growth Factor-basic (rbFGF-basic) produced in E.coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rbFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% by SDS-PAGE analysis.

• Bioactivity:

  ED50 < 1.0 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1 x 10ˆ6 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/mL.

• Molecular Weight:

  16.4 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant bovine Fibroblast Growth Factor-basic (rbFGF-basic) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rbFGF-basic remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  PAL PEDGGSGAFP PGHFKDPKRL YCKNGGFFLR IHPDGRVDGV REKSDPHIKL QLQAEERGVV SIKGVCANRY LAMKEDGRLL ASKCVTDECF FFERLESNNY NTYRSRKYSS WYVALKRTGQ YKLGPKTGPG QKAILFLPMS AKS