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RecombinantFGF-10, Mouse

CAT: 0013-GTR18991791-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18991791-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2) . It shares homology with KGF, and both KGF and FGF-10 activate the receptor FGFR2-IIIb. However, unlike KGF, which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during multi-organ development via mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development and is regulated by Shh during early development. Recombinant mouse Fibroblast Growth Factor-10 (rmFGF-10) produced in E.coli is a single non-glycosylated polypeptide chain containing 148 amino acids. A fully biologically active molecule, rmFGF-10 has a molecular mass of 17.0 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Product Name Alternative
Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2
Source
Escherichia coli.
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE and HPLC.
Bioactivity
ED50 1.0x 10ˆ5 units/mg.
Form
Lyophilized after extensive dialysis against PBS.
Molecular Weight
17.0 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant mouse Fibroblast Growth Factor-10 (rmFGF-10) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-10 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O at 100 μg/mL.
Preservative
Lyophilized after extensive dialysis against PBS.
AA Sequence
SSA GRHVRSYNHL QGDVRWRRLF SFTKYFLTIE KNGKVSGTKN EDCPYSVLEI TSVEIGVVAV KAINSNYYLA MNKKGKLYGS KEFNNDCKLK ERIEENGYNT YASFNWQHNG RQMYVALNGK GAPRRGQKTR RKNTSAHFLP MTIQT

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