RecombinantFGF-21, Mouse

• Description:

  Fibroblast growth factor-21 (FGF21) belongs to the large FGF family which has at least 23 members. Along with FGF-19/15 and FGF-23, FGF-21 is categorized as a member of the atypical FGF subfamily, as it must be complexed to the Klotho co-receptor in order to bind to the FGF receptors and activate the downstream signaling pathway. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Recombinant Mouse Fibroblast Growth Factor-21 (FGF-21) produced in E.coli is a single non-glycosylated polypeptide chain containing 182 amino acids. A fully biologically active molecule, rmFGF-21 has a molecular mass of 19.9 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 97% as analyzed by SDS-PAGE& HPLC.

• Bioactivity:

  ED50 < 0.5 μg/ml, measured by a cell proliferation assay using NIH-3T3 cells in the presence of 1.25 µg/mL mouse Klotho and 10 µg/mL heparin, corresponding to a specific activity of > 2 x 10ˆ3 units/mg.

• Reconstitution:

  Reconstituted in ddH2O or PBS at 100 μg/ml.

• Molecular Weight:

  19.9 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant Mouse Fibroblast Growth Factor-21 (FGF-21), remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse FGF-21 should be stable up to 1 week at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  AYP IPDSSPLLQF GGQVRQRYLY TDDDQDTEAH LEIREDGTVV GAAHRSPESL LELKALKPGV IQILGVKASR FLCQQPDGAL YGSPHFDPEA CSFRELLLED GYNVYQSEAH GLPLRLPQKD SPNQDATSWG PVRFLPMPGL LHEPQDQAGF LPPEPPDVGS SDPLSMVEPL QGRSPSYAS