RecombinantFGF-acidic, Mouse

CAT: 0384-BK0052-01Size: 10 µgDry Ice: NoHazardous: No
CAT#:0384-BK0052-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibroblast Growth Factor- acidic (FGF-acidic) is a mitogen targeting at the endothelial cells, and belongs to the heparin binding FGF family, which contains 22 members. FGF-acidic binds to the receptor family FGFR1-4 in vivo with the assistance of heparin. However, along with FGF -basic, FGF-acidic lacks the signal peptide segment, and thus is not secreted via endoplasmic reticulum (ER) and Golgi bodies. Studies have shown that FGF-acidic is highly regulated, and it is a direct angiogenesis factor. If unregulated, angiogenesis could contribute to several diseases including arthritis, diabetes, ocular neovascularization, and especially tumors. Therefore, FGF-acidic is treated as a potential oncogene, and its overexpression is correlated tightly with several cancers. Recombinant mouse Fibroblast Growth Factor- acidic (rmFGF-acidic) produced in E.coli is a single non-glycosylated polypeptide chain containing 140 amino acids. A fully biologically active molecule, rmFGF-acidic has a molecular mass of 15.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 < 0.4 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 10 µg/mL heparin, corresponding to a specific activity of > 2.5x 10ˆ6 units/mg.
Reconstitution
Reconstituted in ddH2O at 100 μg/mL.
Molecular Weight
15.8 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant mouse Fibroblast Growth Factor- acidic (rmFGF-acidic) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-acidic should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
FNL PLGNYKKPKL LYCSNGGHFL RILPDGTVDG TRDRSDQHIQ LQLSAESAGE VYIKGTETGQ YLAMDTEGLL YGSQTPNEEC LFLERLEENH YNTYTSKKHA EKNWFVGLKK NGSCKRGPRT HYGQKAILFL PLPVSSD

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