RecombinantFGF-acidic, Mouse

• Description:

  Fibroblast Growth Factor- acidic (FGF-acidic) is a mitogen targeting at the endothelial cells, and belongs to the heparin binding FGF family, which contains 22 members. FGF-acidic binds to the receptor family FGFR1-4 in vivo with the assistance of heparin. However, along with FGF -basic, FGF-acidic lacks the signal peptide segment, and thus is not secreted via endoplasmic reticulum (ER) and Golgi bodies. Studies have shown that FGF-acidic is highly regulated, and it is a direct angiogenesis factor. If unregulated, angiogenesis could contribute to several diseases including arthritis, diabetes, ocular neovascularization, and especially tumors. Therefore, FGF-acidic is treated as a potential oncogene, and its overexpression is correlated tightly with several cancers. Recombinant mouse Fibroblast Growth Factor- acidic (rmFGF-acidic) produced in E.coli is a single non-glycosylated polypeptide chain containing 140 amino acids. A fully biologically active molecule, rmFGF-acidic has a molecular mass of 15.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% by SDS-PAGE and HPLC analyses.

• Bioactivity:

  ED50 < 0.4 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 10 µg/mL heparin, corresponding to a specific activity of > 2.5x 10ˆ6 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/mL.

• Molecular Weight:

  15.8 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant mouse Fibroblast Growth Factor- acidic (rmFGF-acidic) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-acidic should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  FNL PLGNYKKPKL LYCSNGGHFL RILPDGTVDG TRDRSDQHIQ LQLSAESAGE VYIKGTETGQ YLAMDTEGLL YGSQTPNEEC LFLERLEENH YNTYTSKKHA EKNWFVGLKK NGSCKRGPRT HYGQKAILFL PLPVSSD