RecombinantCNTF, Mouse

CAT: 0384-BK0018-01Size: 10 µgDry Ice: NoHazardous: No
CAT#:0384-BK0018-01Size:10 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone which acts within the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF is a potent survival factor for neurons and oligodendrocytes and may play a role in reducing tissue damage during increased inflammation. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, however this phenotype is not causally related to neurologic disease. Recombinant Mouse CNTF produced in E.coli is a single, non-glycosylated polypeptide chain of 197 amino acids and a molecular mass of 22.6 kDa. It has been purified by chromatographic techniques.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE.
Bioactivity
ED50 < 30ng/ml, measured by its ability to induce alkaline phosphatase production byTF-1 Cells.
Reconstitution
Reconstituted in ddH2O at 100 μg/ml.
Molecular Weight
22.6 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant Mouse CNTF remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse CNTF should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLD SVDGVPVAS T DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LTLQVSAFA Y QLEELMALLE QKVPEKEADG MPVTIGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVIS S HHMGISAHES HYGAKQM

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