Products for Research Use Only
RegisterLogin

RecombinantCNTF, Human

CAT: 0384-BK0017-01Size: 10 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0384-BK0017-01Size:10 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Ciliary Neurotrophic Factor (CNTF) is a cytokine belonging to the Interleukin 6 (IL-6) family, which also includes IL-6, Oncostatin M, Leukemia Inhibitory Factor (LIF), and Cardiotrophin-1. Structurally, CNTF resembles a four-helix bundle composition, similar to the other members of the IL-6 family. The receptor for CNTF is composed of three parts: a gp130-like subunit common in the IL-6 receptor family, a LIF Receptor β subunit, and a CNTF specific α receptor subunit. Upon binding to the CNTF, the β subunit of the CNTF receptor will undergo tyrosine phosphorylation, and activate the intracellular JAK/STAT pathway. The main function of CNTF in vivo is to promote the differentiation and survival of a variety of neurons and glial cells, including sympathetic precursor cells and spinal motor neurons.Recombinant human Ciliary Neurotrophic Factor (rhCNTF) produced in E. coli is a single non-glycosylated polypeptide chain containing 199 amino acids. A fully biologically active molecule, rhCNTF has a molecular mass of 22.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE and HPLC.
Bioactivity
ED50 < 200 ng/ml, measured cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 5x10ˆ3 units/mg.
Reconstitution
Reconstituted in ddH2O or PBS at 100 μg/ml.
Molecular Weight
22.8 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant human Ciliary Neurotrophic Factor (rhCNTF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhCNTF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
AFT EHSPLTPHRR DLCSRSIWLA RKIRSDLTAL TESYVKHQGL NKNINLDSAD GMPVASTDQW SELTEAERLQ ENLQAYRTFH VLLARLLEDQ QVHFTPTEGD FHQAIHTLLL QVAAFAYQIE ELMILLEYKI PRNEADGMPI NVGDGGLFEK KLWGLKVLQE LSQWTVRSIH DLRFISSHQT GIPARGSHYI ANNKKM

Related Products

CatalogName
BK0018-01RecombinantCNTF, Mouse
BK0019-01RecombinantCNTF, Rat
BK0171-01RecombinantTNF-α, Human
BK0144-01RecombinantNT-4, Human
BK0191-01RecombinantCT-1, Human
BK0169-01RecombinantTNF-α, His, Human
BK0347-01RecombinantTNF-α, Human (P.pastoris-expressed)
BK0168-01RecombinantTNF-α, Bovine
BK0301-01RecombinantCT‑1, Mouse
BK0349-01RecombinantTNF-α, Rat

Alternative Products

CatalogName
GTR18991957-01RecombinantCNTF, Human
BK0017RecombinantCNTF,Human
GTR18990242-01RecombinantCNTF, Human (HEK293-expressed)
BK0300RecombinantCNTF,Human(HEK293-expressed)
BK0018RecombinantCNTF,Mouse
GTR18991946-01RecombinantCNTF, Mouse
GTR18991935-01RecombinantCNTF, Rat
BK0019RecombinantCNTF,Rat
P83251PMG36e-ADH1B (human-opt-human) -ALDH2 (human-opt-human)
P81559PCMV-Twinstrep-UFM1 (human) -UFM1 (human-opt-human) -UFM1 (human-opt-human) -Neo

Popular Products