RecombinantNT-4, Mouse

• Description:

  Neurotrophin-4 (NT-4) is a small secreted cytokine, and belongs to the Neurotrophin (NT) family, which also includes Brain Derived Neurotropic Factor (BDNF), Nerve Growth Factor (NGF), and NT-3. NT family members are all derived from similar sized protein precursors, composed of N-terminal propeptides and C-terminal mature domains, which are separated by posttranslational proteolytic cleavage. NT-4 (along with NT-3) is foundin the brains of mammals . In vivo, NT-4 binds to the common receptor, p75NTR, and a tyrosine kinase receptor, TrkB. The heterotrimeric complex activates the NFκB transcription factor. NT-4 is essential for the differentiation and wiring regulation of the central and peripheral nervous systems during development, and is related to important diseases including Alzheimer’s.Recombinant mouse Neurotrophin-4 (rmNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chainsof 131 amino acids. A fully biologically active molecule, rmNT-4 has a molecular mass of 14.0kDaanalyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% by SDS-PAGEanalysis.

• Bioactivity:

  ED50 <1µg/mL, measured by a cell proliferation assay usingC6cells, corresponding to a specific activity of>1x 10ˆ3 units/mg.

• Reconstitution:

  Reconstituted in 50mM acetic acid or ddH2O at 100 μg/mL.

• Molecular Weight:

  14.0 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant mouse Neurotrophin-4 (rmNT-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmNT-4 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against 50mM acetic acid.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  MGVSETAPA SRRGELAVCD AVSGWVTDRR TAVDLRGREV EVLGEVPAAG GSPLRQYFFE TRCKAESAGE GGPGVGGGGC RGVDRRHWLS ECKAKQSYVR ALTADSQGRV GWRWIRIDTA CVCTLLSRTG RA