RecombinantTNF-α, His, Mouse

• Description:

  Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-alpha) produced in E. coli is a non-glycosylated polypeptide chain of 163 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 18.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

• Endotoxin:

  <1 EU/μg, determined by LAL method.

• Purity:

  > 95% as analyzed by reducing SDS-PAGE.

• Bioactivity:

  ED50<0.05ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 2x 10ˆ7units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/ml.

• Molecular Weight:

  18.3kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-slpha) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rmTNF-alpha should be stable up to 4 week at 4°C or up to 6 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  MHHHHHHML RSSSQNSSDK PVAHVVANHQ VEEQLEWLSQ RANALLANGM DLKDNQLVVP ADGLYLVYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVN LLSAVKSPCP KDTPEGAELK PWYEPIYLGG VFQLEKGDQL SAEVNLPKYL DFAESGQVYF GVIAL