Piccolo Antibody

• Background:

  Piccolo (also known as Aczonin) is a large, multidomain scaffolding protein localized at the presynaptic active zone. It plays a central role in organizing the cytomatrix at active zones (CAZ) and coordinating synaptic vesicle trafficking, exocytosis, and endocytosis. Piccolo interacts with proteins involved in actin dynamics and clathrin-mediated endocytosis, including Abp1 and dynamin, thereby regulating synaptic vesicle recycling. It is often co-expressed with Bassoon, another CAZ protein, to maintain synaptic integrity and plasticity. Disruption of Piccolo expression or function has been linked to neurodevelopmental and neurodegenerative disorders, including schizophrenia and Parkinson’s disease. Its role in synaptic maintenance and plasticity makes Piccolo a promising target for understanding and treating synaptic dysfunction in neurological diseases.

• Description:

  Rabbit Anti-Human Piccolo Polyclonal

• Specifications:

  Detects ~550kDa. No cross-reactivity to Bassoon.

• Product Name Alternative:

  Protein piccolo, Aczonin, PCLO, Presynaptic cytomatrix protein, Multidomain presynaptic cytomatrix protein, Piccolo (presynaptic cytomatrix protein), PCLO_HUMAN, ACZ, Pico, Brain-derived HLMN protein, DKFZp779G1236, KIAA0559, Piccolo mouse homolog of Pico

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  27445

• Swiss Prot:

  Q9Y6V0

• Accession Number:

  NP_055325.2

• Cellular Locus:

  Cell Junction | Synapse

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant Full length human Piccolo Protein, BC001304.1

• Target:

  Piccolo

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal

• Applications:

  WB | IHC

• Validated Applications:

  WB, IHC

• Field of Research:

  Neuroscience | Cell Markers | Neuron Markers | Presynaptic Markers

• Purification:

  Protein A Purified

• Detection Range:

  1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:400) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20 (7) : 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147 (1) : 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281 (9) :6038-6047.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects ~550kDa. No cross-reactivity to Bassoon.

• Species:

  Human | Mouse | Rat

• CAS Number:

  9007-83-4

• Location:

  Cell Junction | Synapse

• Immunogen Species:

  Human